Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rose, J.R.; Salto, R.; Craik, C.S.
    Regulation of autoproteolysis of the HIV-1 and HIV-2 proteases with engineered amino acid substitutions (1993), J. Biol. Chem., 268, 11939-11945.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.23.47 medicine potential target for chemotherapy of virus infection and associated diseases, essential for maturation of infectious virions, development of drugs against the protease should be effective against HIV-2 Human immunodeficiency virus 2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.47 cloned and expressed in Escherichia coli JM-101, F' and X90 Human immunodeficiency virus 2

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.47 crystallized by hanging drop method in complex with a reduced amide inhibitor, BI-LA-398 Human immunodeficiency virus 2

General Stability

EC Number General Stability Organism
3.4.23.47 activity is lost during purification and storage Human immunodeficiency virus 2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.47 0.74
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C Human immunodeficiency virus 2
3.4.23.47 0.95
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C, mutant K7Q Human immunodeficiency virus 2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.47 GAG precursor protein of HIV-2 + H2O Human immunodeficiency virus 2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.47 Human immunodeficiency virus 2
-
 mutant K7Q
-
3.4.23.47 Human immunodeficiency virus 2
-
 human immunodeficiency virus type 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.47 mutant K7Q, autoproteolysis Human immunodeficiency virus 2
3.4.23.47 recombinant enzyme Human immunodeficiency virus 2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.47 GAG precursor protein of HIV-2 + H2O
-
 Human immunodeficiency virus 2 ?
-
 ?
3.4.23.47 additional information cleavage sites identified by amino-terminal sequence analysis are F3-S4, Y14-I15, G35-I36, G52-F53, M76-T77, L90-T91 Human immunodeficiency virus 2 ?
-
 ?
3.4.23.47 NH2-ATLNFPSPW-COOH + H2O
-
 Human immunodeficiency virus 2 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.47 3
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C Human immunodeficiency virus 2
3.4.23.47 4.33
-
 NH2-ATLNFPSPW-COOH pH 5.5, 37°C, mutant K7Q Human immunodeficiency virus 2